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%%EOF The experimental approach is essentially identical to that described earlier for an acidbase titration, to which you may refer. At the equivalence point all the Cd2+ initially in the titrand is now present as CdY2. $d 7$ 8$ H$ a$gd, d 7$ 8$ H$ gd% | ~ zhY h, 5CJ OJ QJ ^J aJ #h, h, 5CJ OJ QJ ^J aJ #h, h% 5CJ OJ QJ ^J aJ +h;- h, 5CJ OJ QJ ^J aJ mHsH.h;- h% 5CJ H*OJ QJ ^J aJ mHsH +h;- h% 5CJ OJ QJ ^J aJ mHsH.h;- h, 5CJ H*OJ QJ ^J aJ mHsH .h;- h% 5CJ H*OJ QJ ^J aJ mHsH q t xcM8 (h, h% CJ# OJ QJ ^J aJ# mHsH +h In addition, EDTA must compete with NH3 for the Cd2+. It is unfit for drinking, bathing, washing and it also forms scales in Thus one simply needs to determine the area under the curve of the unknown and use the calibration curve to find the unknown concentration. Perform calculations to determine the concentration of calcium and magnesium ions in the hard water. 0000005100 00000 n If at least one species in a complexation titration absorbs electromagnetic radiation, we can identify the end point by monitoring the titrands absorbance at a carefully selected wavelength. If preparation of such sample is difficult, we can use different EDTA concentration. One consequence of this is that the conditional formation constant for the metalindicator complex depends on the titrands pH. Figure 9.32 End point for the titration of hardness with EDTA using calmagite as an indicator; the indicator is: (a) red prior to the end point due to the presence of the Mg2+indicator complex; (b) purple at the titrations end point; and (c) blue after the end point due to the presence of uncomplexed indicator. \end{align}\], Substituting into equation 9.14 and solving for [Cd2+] gives, \[\dfrac{[\mathrm{CdY^{2-}}]}{C_\textrm{Cd}C_\textrm{EDTA}} = \dfrac{3.13\times10^{-3}\textrm{ M}}{C_\textrm{Cd}(6.25\times10^{-4}\textrm{ M})} = 9.5\times10^{14}\], \[C_\textrm{Cd}=5.4\times10^{-15}\textrm{ M}\], \[[\mathrm{Cd^{2+}}] = \alpha_\mathrm{Cd^{2+}} \times C_\textrm{Cd} = (0.0881)(5.4\times10^{-15}\textrm{ M}) = 4.8\times10^{-16}\textrm{ M}\]. Because the pH is 10, some of the EDTA is present in forms other than Y4. If MInn and Inm have different colors, then the change in color signals the end point. \end{align}\]. EDTA (L) Molarity. 4! The next task in calculating the titration curve is to determine the volume of EDTA needed to reach the equivalence point. EDTA (mol / L) 1 mol Calcium. In this section we will learn how to calculate a titration curve using the equilibrium calculations from Chapter 6. As shown in the following example, we can easily extended this calculation to complexation reactions using other titrants. Solution for Calculate the % Copper in the alloy using the average titration vallue. This can be analysed by complexometric titration. MgSO4 Mg2++SO42- Experimental: When the reaction between the analyte and titrant is complete, you can observe a change in the color of the solution or pH changes. where Kf is a pH-dependent conditional formation constant. The quantitative relationship between the titrand and the titrant is determined by the stoichiometry of the titration reaction. Complexation titrimetry continues to be listed as a standard method for the determination of hardness, Ca2+, CN, and Cl in waters and wastewaters. A buffer solution is prepared for maintaining the pH of about 10. Click here to review your answer to this exercise. \end{align}\], To calculate the concentration of free Cd2+ we use equation 9.13, \[[\mathrm{Cd^{2+}}] = \alpha_\mathrm{Cd^{2+}} \times C_\textrm{Cd} = (0.0881)(3.64\times10^{-4}\textrm{ M})=3.21\times10^{-4}\textrm{ M}\], \[\textrm{pCd}=-\log[\mathrm{Cd^{2+}}]=-\log(3.21\times10^{-4}) = 3.49\]. 0000022889 00000 n First, we add a ladder diagram for the CdY2 complex, including its buffer range, using its logKf value of 16.04. Add 12 drops of indicator and titrate with a standard solution of EDTA until the red-to-blue end point is reached (Figure 9.32). The sample, therefore, contains 4.58104 mol of Cr. Although each method is unique, the following description of the determination of the hardness of water provides an instructive example of a typical procedure. of standard calcium solution are assumed equivalent to 7.43 ml. endstream endobj 244 0 obj <>/Metadata 80 0 R/Pages 79 0 R/StructTreeRoot 82 0 R/Type/Catalog/ViewerPreferences<>>> endobj 245 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]>>/Rotate 0/StructParents 0/TrimBox[0.0 0.0 595.276 841.89]/Type/Page>> endobj 246 0 obj <> endobj 247 0 obj <>stream \[\alpha_{\textrm Y^{4-}} \dfrac{[\textrm Y^{4-}]}{C_\textrm{EDTA}}\tag{9.11}\]. For the titration of Mg2+, one must buffer the solution to a pH of 10 so that complex formation will be quantitative. For example, an NH4+/NH3 buffer includes NH3, which forms several stable Cd2+NH3 complexes. %PDF-1.4 % 1 Answer anor277 . Calculate the total millimoles of aluminum and magnesium ions in the antacid sample solution and in the tablet. The analogous result for a complexation titration shows the change in pM, where M is the metal ion, as a function of the volume of EDTA. 0 2 4 seWEeee #hLS h% CJ H*OJ QJ ^J aJ hLS CJ OJ QJ ^J aJ hp CJ OJ QJ ^J aJ h`. Endpoints in the titration are detected using. Compare your results with Figure 9.28 and comment on the effect of pH and of NH3 on the titration of Cd2+ with EDTA. Magnesium. Water hardness is determined by the total concentration of magnesium and calcium. Because the calculation uses only [CdY2] and CEDTA, we can use Kf instead of Kf; thus, \[\dfrac{[\mathrm{CdY^{2-}}]}{[\mathrm{Cd^{2+}}]C_\textrm{EDTA}}=\alpha_\mathrm{Y^{4-}}\times K_\textrm f\], \[\dfrac{3.13\times10^{-3}\textrm{ M}}{[\mathrm{Cd^{2+}}](6.25\times10^{-4}\textrm{ M})} = (0.37)(2.9\times10^{16})\]. To calculate magnesium solution concentration use EBAS - stoichiometry calculator. We begin by calculating the titrations equivalence point volume, which, as we determined earlier, is 25.0 mL. Report the purity of the sample as %w/w NaCN. Otherwise, the calcium will precipitate and either you'll have no endpoint or a weak endpoint. The titration can be carried out with samples with chloride contents of a few ppm - 100%, but the amount of sample has to be adjusted. concentration and the tap water had a relatively normal level of magnesium in comparison. Calculate the Aluminum hydroxide and Magnesium hydroxide content in grams in the total diluted sample. Because the color of calmagites metalindicator complex is red, its use as a metallochromic indicator has a practical pH range of approximately 8.511 where the uncomplexed indicator, HIn2, has a blue color. Background Calcium is an important element for our body. Figure 9.30 (a) Predominance diagram for the metallochromic indicator calmagite showing the most important form and color of calmagite as a function of pH and pMg, where H2In, HIn2, and In3 are uncomplexed forms of calmagite, and MgIn is its complex with Mg2+. The reaction of Mg2+ with EDTA may be expressed as: Mg2+ + H2Y2- = MgY-2 + 2H+ The structure of EDTA and the magnesium-EDTA complex (without the hydrogen atoms) is shown below: The endpoint of the titration is determined by the . 0000024212 00000 n Erlenmeyer flask. mH nH uh7 j h7 Uh j h U h)v h0Z CJ OJ QJ ^J aJ h, CJ OJ QJ ^J aJ hB CJ OJ QJ ^J aJ hZ7 CJ OJ QJ ^J aJ Uh0Z CJ OJ QJ ^J aJ h)v CJ OJ QJ ^J aJ hp CJ OJ QJ ^J aJ f charge attraction. The second titration uses, \[\mathrm{\dfrac{0.05831\;mol\;EDTA}{L}\times0.03543\;L\;EDTA=2.066\times10^{-3}\;mol\;EDTA}\]. Because the reactions formation constant, \[K_\textrm f=\dfrac{[\textrm{CdY}^{2-}]}{[\textrm{Cd}^{2+}][\textrm{Y}^{4-}]}=2.9\times10^{16}\tag{9.10}\]. Solving gives [Cd2+] = 4.71016 M and a pCd of 15.33. CJ OJ QJ ^J aJ h`. In this study \end{align}\], \[\begin{align} +h;- h% 5CJ OJ QJ ^J aJ mHsHhs CJ OJ QJ ^J aJ h, CJ OJ QJ ^J aJ #hs h% CJ H*OJ QJ ^J aJ h, h% CJ OJ QJ ^J aJ h, h% CJ OJ QJ ^J aJ hk h% CJ OJ QJ ^J aJ &h, h% 5CJ H*OJ QJ ^J aJ &h, h% 5CJ H*OJ QJ ^J aJ #h, h% 5CJ OJ QJ ^J aJ h, 5CJ OJ QJ ^J aJ v x F  n o d 7$ 8$ H$ ^`gd Because not all the unreacted Cd2+ is freesome is complexed with NH3we must account for the presence of NH3. Titrating with 0.05831 M EDTA required 35.43 mL to reach the murexide end point. The other three methods consisted of direct titrations (d) of mangesium with EDTA to the EBT endpoint after calcium had been removed. C_\textrm{EDTA}&=\dfrac{M_\textrm{EDTA}V_\textrm{EDTA}-M_\textrm{Cd}V_\textrm{Cd}}{V_\textrm{Cd}+V_\textrm{EDTA}}\\ With respect to #"magnesium carbonate"#, this is #17 . The concentration of a solution of EDTA was determined by standardizing against a solution of Ca2+ prepared using a primary standard of CaCO3. See Chapter 11 for more details about ion selective electrodes. The same unknown which was titrated will be analyzed by IC. nzRJq&rmZA /Z;OhL1. From the chromatogram it is possible to get the area under the curve which is directly related to the concentration of the analyte. Dilutes with 100 ml of water and titrate the liberated iodine with 0.1M sodium thiosulphate using 0.5ml of starch solution, added towards the end of the titration, as an indicator. See Figure 9.11 for an example. Table 9.12 provides values of M2+ for several metal ion when NH3 is the complexing agent. Perform a blank determination and make any necessary correction. Ethylenediaminetetraacetate (EDTA) complexes with numerous mineral ions, including calcium and magnesium. EDTA (mol / L) 1 mol Magnesium. After the equivalence point the absorbance remains essentially unchanged. 21 19 Complexation Titration is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by LibreTexts. Before adding EDTA, the mass balance on Cd2+, CCd, is, and the fraction of uncomplexed Cd2+, Cd2+, is, \[\alpha_{\textrm{Cd}^{2+}}=\dfrac{[\mathrm{Cd^{2+}}]}{C_\textrm{Cd}}\tag{9.13}\]. Repeat the titration twice. h`. A pH indicatorxylene cyanol FFis added to ensure that the pH is within the desired range. Dilute 20ml of the sample in Erlenmeyer flask to 40ml by adding 20ml of distilled water. The molarity of EDTA in the titrant is, \[\mathrm{\dfrac{4.068\times10^{-4}\;mol\;EDTA}{0.04263\;L\;EDTA} = 9.543\times10^{-3}\;M\;EDTA}\]. The sample was acidified and titrated to the diphenylcarbazone end point, requiring 6.18 mL of the titrant. Let the burette reading of EDTA be V 2 ml. ), The primary standard of Ca2+ has a concentration of, \[\dfrac{0.4071\textrm{ g CaCO}_3}{\textrm{0.5000 L}}\times\dfrac{\textrm{1 mol Ca}^{2+}}{100.09\textrm{ g CaCO}_3}=8.135\times10^{-3}\textrm{ M Ca}^{2+}\], \[8.135\times10^{-3}\textrm{ M Ca}^{2+}\times0.05000\textrm{ L Ca}^{2+} = 4.068\times10^{-4}\textrm{ mol Ca}^{2+}\], which means that 4.068104 moles of EDTA are used in the titration. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright . Eriochrome Black-T(EBT) is the metal ion indicator used in the determination of hardness by complexometric titration with EDTA. U! Procedure for calculation of hardness of water by EDTA titration. Table 2 Determination of Total Hardness of Water Trials Volume of Sample (mL) Nt. H|W$WL-_ |`J+l$gFI&m}}oaQfl%/|}8vP)DV|{*{H [1)3udN{L8IC 6V ;2q!ZqRSs9& yqQi.l{TtnMIrW:r9u$ +G>I"vVu/|;G k-`Jl_Yv]:Ip,Ab*}xqd e9:3x{HT8| KR[@@ZKRS1llq=AE![3 !pb If the metalindicator complex is too strong, the change in color occurs after the equivalence point. Record the volume used (as V.). seems!to!proceed!slowly!near!the!equivalence!point,!after!each!addition!of! The description here is based on Method 2340C as published in Standard Methods for the Examination of Water and Wastewater, 20th Ed., American Public Health Association: Washington, D. C., 1998. The determination of the Calcium and Magnesium next together in water is done by titration with the sodium salt of ethylenediaminetetraethanoic acid (EDTA) at pH 8 9, the de- tection is carried out with a Ca electrode. Because Ca2+ forms a stronger complex with EDTA, it displaces Mg2+, which then forms the red-colored Mg2+calmagite complex. Hardness is mainly the combined constituent of both magnesium and calcium. Titration is one of the common method used in laboratories which determines the unknown concentration of an analyte that has been identified. For example, after adding 5.0 mL of EDTA, the total concentration of Cd2+ is, \[\begin{align} Calculate the %w/w Na2SO4 in the sample. In 1945, Schwarzenbach introduced aminocarboxylic acids as multidentate ligands. Figure 9.27 shows a ladder diagram for EDTA. An analysis done on a series of samples with known concentrations is utilized to build a calibration curve. Currently, titration methods are the most common protocol for the determination of water hardness, but investigation of instrumental techniques can improve efficiency. At any pH a mass balance on EDTA requires that its total concentration equal the combined concentrations of each of its forms. a mineral analysis is performed, hardness by calculation can be reported. Standardization of EDTA: 20 mL of the standard magnesium sulfate solution is pipetted out into a 250 mL Erlenmeyer flask and diluted to 100 mL . Let the burette reading of EDTA be V 3 ml. EDTA and the metal ion in a 1:1 mole ratio. Magnesium ions form a less stable EDTA complex compared to calcium ions but a more stable indicator complex hence a small amount of Mg2+ or Mg-EDTA complex is added to the reaction mixture during the titration of Ca2+ with EDTA. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. Titrating with EDTA using murexide or Eriochrome Blue Black R as the indicator gives the concentration of Ca2+. The solid lines are equivalent to a step on a conventional ladder diagram, indicating conditions where two (or three) species are equal in concentration. (Assume the moles of EDTA are equal to the moles of MgCO3) Chemistry Reactions in Solution Titration Calculations. last modified on October 27 2022, 21:28:28. 21 0 obj <> endobj \[\textrm{MIn}^{n-}+\textrm Y^{4-}\rightarrow\textrm{MY}^{2-}+\textrm{In}^{m-}\]. End point of magnesium titration is easily detected with Eriochrome BlackT. To perform titration we will need titrant - 0.01M EDTA solution and ammonia pH10.0 buffer. 8. This shows that the mineral water sample had a relatively high. 0000021941 00000 n Reaction taking place during titration is. endstream endobj 267 0 obj <>/Filter/FlateDecode/Index[82 161]/Length 27/Size 243/Type/XRef/W[1 1 1]>>stream h, 5>*CJ H*OJ QJ ^J aJ mHsH.h Detection is done using a conductivity detector. The amount of calcium present in the given sample can be calculated by using the equation. 0000023545 00000 n 243 0 obj <> endobj hb``c``ie`a`p l@q.I7!$1)wP*Sy-+]Ku4y^TQP h Q2qq 8LJb2rO.dqukR Cp/N8XbS0X_.fhhbCKLg4o\4i uB The stoichiometry between EDTA and each metal ion is 1:1. An alloy of chromel containing Ni, Fe, and Cr was analyzed by a complexation titration using EDTA as the titrant. A 100.0-mL sample is analyzed for hardness using the procedure outlined in Representative Method 9.2, requiring 23.63 mL of 0.0109 M EDTA. Calmagite is used as an indicator. Reactions taking place startxref (Use the symbol Na 2 H 2 Y for Na 2 EDTA.) Correcting the absorbance for the titrands dilution ensures that the spectrophotometric titration curve consists of linear segments that we can extrapolate to find the end point. A similar calculation should convince you that pCd = logKf when the volume of EDTA is 2Veq. Our derivation here is general and applies to any complexation titration using EDTA as a titrant. C_\textrm{Cd}&=\dfrac{\textrm{initial moles Cd}^{2+} - \textrm{moles EDTA added}}{\textrm{total volume}}=\dfrac{M_\textrm{Cd}V_\textrm{Cd}-M_\textrm{EDTA}V_\textrm{EDTA}}{V_\textrm{Cd}+V_\textrm{EDTA}}\\ The titration is done with 0.1 mol/l AgNO3 solution to an equivalence point. A late end point and a positive determinate error are possible if we use a pH of 11. the reason for adding Mg-EDTA complex as part of the NH 4 Cl - NH 4 OH system explained in terms of requirement of sufficient inactive Mg2+ ions to provide a sharp colour change at the endpoint. Standard magnesium solution, 0.05 M. Dissolve 1.216 g of high purity mag- nesium (Belmont 99.8%) in 200 ml of 20% hydrochloric acid and dilute to 11. 0000001283 00000 n Because EDTA has many forms, when we prepare a solution of EDTA we know it total concentration, CEDTA, not the concentration of a specific form, such as Y4. Add 10 mL of ammonia buffer, 50 mL of distilled water and 1 mL of Eriochrome Black T indicator The Titration After the magnesium ions have been precipitated out of the hard water by the addition of NaOH (aq) to form white Mg(OH) 2(s), the remaining Ca 2+ ions in solution are titrated with EDTA solution.. Determination of Hardness: Hardness is expressed as mg/L CaCO 3. The method adopted for the Ca-mg analysis is the complexometric titration. For example, after adding 30.0 mL of EDTA, \[\begin{align} Complexation titrations, however, are more selective. Some!students! Step 2: Calculate the volume of EDTA needed to reach the equivalence point. 4 Sample Calculations (Cont.) Beginning with the conditional formation constant, \[K_\textrm f'=\dfrac{[\mathrm{CdY^{2-}}]}{[\mathrm{Cd^{2+}}]C_\textrm{EDTA}}=\alpha_\mathrm{Y^{4-}} \times K_\textrm f = (0.37)(2.9\times10^{16})=1.1\times10^{16}\], we take the log of each side and rearrange, arriving at, \[\log K_\textrm f'=-\log[\mathrm{Cd^{2+}}]+\log\dfrac{[\mathrm{CdY^{2-}}]}{C_\textrm{EDTA}}\], \[\textrm{pCd}=\log K_\textrm f'+\log\dfrac{C_\textrm{EDTA}}{[\mathrm{CdY^{2-}}]}\]. What problems might you expect at a higher pH or a lower pH? (i) Calculation method For this method, concentration of cations should be known and then all concentrations are expressed in terms of CaCO 3 using Eq. The resulting metalligand complex, in which EDTA forms a cage-like structure around the metal ion (Figure 9.26b), is very stable. Each ml of 0.1M sodium thiosulphate is equivalent to 0.02703 g of FeCI3,6H2O. mole( of( EDTA4-perliter,and&VEDTA( is( the( volume( of EDTA 4- (aq)inunitsofliter neededtoreachtheendpoint.If( you followed instructions, V Mg =0.025Land( C EDTA =( This is the same example that we used in developing the calculations for a complexation titration curve. HWM6W- ~jgvuR(J0$FC*$8c HJ9b\I_~wfLJlduPl Even if a suitable indicator does not exist, it is often possible to complete an EDTA titration by introducing a small amount of a secondary metalEDTA complex, if the secondary metal ion forms a stronger complex with the indicator and a weaker complex with EDTA than the analyte. Table 9.13 and Figure 9.28 show additional results for this titration. Prepare a standard solution of magnesium sulfate and titrate it against the given EDTA solution using Eriochrome Black T as the indicator. \[\begin{align} Your TA will give you further information on how you will obtain your data. A 0.4071-g sample of CaCO3 was transferred to a 500-mL volumetric flask, dissolved using a minimum of 6 M HCl, and diluted to volume. ! The next task in calculating the titration curve is to determine the volume of EDTA needed to reach the equivalence point. The excess EDTA is then titrated with 0.01113 M Mg2+, requiring 4.23 mL to reach the end point. For example, calmagite gives poor end points when titrating Ca2+ with EDTA. The mean corrected titration volume was 12.25 mL (0.01225 L). In the method described here, the titrant is a mixture of EDTA and two indicators. EDTA. Figure 9.29a shows the result of the first step in our sketch. Titre Vol of EDTA to Neutralise (mls) 1 21. 2ml of serum contains Z mg of calcium. 0000001090 00000 n After filtering and rinsing the precipitate, it is dissolved in 25.00 mL of 0.02011 M EDTA. 5CJ OJ QJ ^J aJ #h`. Cyanide is determined at concentrations greater than 1 mg/L by making the sample alkaline with NaOH and titrating with a standard solution of AgNO3, forming the soluble Ag(CN)2 complex. There are 3 steps to determining the concentration of calcium and magnesium ions in hard water using the complexometric titration method with EDTA: Make a standard solution of EDTA. If we adjust the pH to 3 we can titrate Ni2+ with EDTA without titrating Ca2+ (Figure 9.34b). As we add EDTA it reacts first with free metal ions, and then displaces the indicator from MInn. Because not all the unreacted Cd2+ is freesome is complexed with NH3we must account for the presence of NH3. After transferring a 50.00-mL portion of this solution to a 250-mL Erlenmeyer flask, the pH was adjusted by adding 5 mL of a pH 10 NH3NH4Cl buffer containing a small amount of Mg2+EDTA. What is pZn at the equivalence point? ^.FF OUJc}}J4 z JT'e!u3&. EDTA is a versatile titrant that can be used to analyze virtually all metal ions. As is the case with acidbase titrations, we estimate the equivalence point of a complexation titration using an experimental end point. 0000041216 00000 n 0000020364 00000 n 2. The reaction between Cl and Hg2+ produces a metalligand complex of HgCl2(aq). A variety of methods are available for locating the end point, including indicators and sensors that respond to a change in the solution conditions. A 0.50 g of sample was heated with hydrochloric acid for 10 min. The concentration of Cl in a 100.0-mL sample of water from a freshwater aquifer was tested for the encroachment of sea water by titrating with 0.0516 M Hg(NO3)2. Download determination of magnesium reaction file, open it with the free trial version of the stoichiometry calculator. 5 22. It is a method used in quantitative chemical analysis. Pipette 10 mL of the sample solution into a conical flask. Add a pinch of Eriochrome BlackT ground with sodium chloride (100mg of indicator plus 20g of analytical grade NaCl). This reagent can forms a stable complex with the alkaline earth metal like calcium ion and magnesium ion in alkaline condition pH above 9.0. In addition to its properties as a ligand, EDTA is also a weak acid. This means that the same concentration of eluent is always pumped through the column. h`. The indicator, Inm, is added to the titrands solution where it forms a stable complex with the metal ion, MInn. Note that after the equivalence point, the titrands solution is a metalligand complexation buffer, with pCd determined by CEDTA and [CdY2]. Although many quantitative applications of complexation titrimetry have been replaced by other analytical methods, a few important applications continue to be relevant. Most indicators for complexation titrations are organic dyesknown as metallochromic indicatorsthat form stable complexes with metal ions. 0000002315 00000 n Click Use button. This provides some control over an indicators titration error because we can adjust the strength of a metalindicator complex by adjusted the pH at which we carry out the titration. Recall that an acidbase titration curve for a diprotic weak acid has a single end point if its two Ka values are not sufficiently different. At the beginning of the titration the absorbance is at a maximum. 0000021829 00000 n The value of Cd2+ depends on the concentration of NH3. Click n=CV button above EDTA4+ in the input frame, enter volume and concentration of the titrant used. 0000001334 00000 n This leaves 8.50104 mol of EDTA to react with Cu and Cr. 2. Figure 9.35 Spectrophotometric titration curve for the complexation titration of a mixture of two analytes. How do you calculate EDTA titration? Standardize against pure zinc (Bunker Hill 99.9985%) if high purity magnesium is not available. Solving equation 9.11 for [Y4] and substituting into equation 9.10 for the CdY2 formation constant, \[K_\textrm f =\dfrac{[\textrm{CdY}^{2-}]}{[\textrm{Cd}^{2+}]\alpha_{\textrm Y^{4-}}C_\textrm{EDTA}}\], \[K_f'=K_f\times \alpha_{\textrm Y^{4-}}=\dfrac{[\mathrm{CdY^{2-}}]}{[\mathrm{Cd^{2+}}]C_\textrm{EDTA}}\tag{9.12}\].